Regulation of DNA Replication Licensing and Re-Replication by Cdt1
نویسندگان
چکیده
منابع مشابه
Regulation of DNA Replication Origin Licensing
DNA replication is a fundamental biological process that serves to create two copies of the genetic material during each cell division. Complete and precise replication enables identical sets of genes to be faithfully delivered to daughter cells during each cell division. To achieve rapid duplication of the entire genome, eukaryotic cells initiate DNA replication at multiple locations on each c...
متن کاملRegulation of MCM7 DNA Replication Licensing Activity
Miniature chromosome maintenance (MCM) proteins were initially identified from autonomously replicating sequence in Saccharomyces cerevisiae. Mutations of some of these proteins such as MCM7 or MCM3 in yeast result in loss of the large chunk of yeast chromosomes. MCM7 cDNA encodes a 543-amino acid protein and is ubiquitously expressed in all tissues. Initiation of DNA replication is a complex p...
متن کاملFunctional domains of the Xenopus replication licensing factor Cdt1
During late mitosis and early G1, replication origins are licensed for subsequent replication by loading heterohexamers of the mini-chromosome maintenance proteins (Mcm2-7). To prevent re-replication of DNA, the licensing system is down-regulated at other cell cycle stages. A small protein called geminin plays an important role in this down-regulation by binding and inhibiting the Cdt1 componen...
متن کاملEssential role of human CDT1 in DNA replication and chromatin licensing.
Formation of pre-replicative complexes at origins is an early cell cycle event essential for DNA duplication. A large body of evidence supports the notion that Cdc6 protein, through its interaction with the origin recognition complex, is required for pre-replicative complex assembly by loading minichromosome maintenance proteins onto DNA. In fission yeast and Xenopus, this reaction known as the...
متن کاملCdt1 downregulation by proteolysis and geminin inhibition prevents DNA re-replication in Xenopus.
In late mitosis and G1, Mcm2-7 are assembled onto replication origins to 'license' them for initiation. At other cell cycle stages, licensing is inhibited, thus ensuring that origins fire only once per cell cycle. Three additional factors--the origin recognition complex, Cdc6 and Cdt1--are required for origin licensing. We examine here how licensing is regulated in Xenopus egg extracts. We show...
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ژورنال
عنوان ژورنال: International Journal of Molecular Sciences
سال: 2021
ISSN: 1422-0067
DOI: 10.3390/ijms22105195